Priming alleviates high temperature induced oxidative DNA damage and repair using Apurinic/apyrimidinic endonuclease (Ape1L) homologue in wheat (Triticum aestivum L.)

Crop plants require an optimum range of temperature for normal growth and development however high temperature can adversely affect the plants, induce oxidative stress and disintegrate biomolecules especially DNA and proteins. In wheat, high temperature stress (35-40 °C) during ripening stage hampers the yield tremendously. In this study, we assessed high temperature (HT) induced oxidative stress, subsequent DNA damage and role of priming in stress tolerance by analyzing DNA repair enzyme Triticum aestivum AP endonuclease (TaApe1L). Sixteen days old seedlings of wheat varieties PBW 550 and PBW 343 were primed with mild drought and exposed to HT (38 °C) for 2, 4, and 6 h. Hydrogen peroxide (H₂O₂) was used as oxidative stress marker and quantified on regular time intervals. DNA damage was analyzed by DNA laddering and TaApe1L gene expression was analyzed using RT PCR and western blotting. Phylogenetic analysis of Ape1 revealed presence of some key amino acids that are evolutionary conserved. A significant increase in H₂O₂ content was observed after 6 h of exposure especially in PBW 343. Similarly, the DNA damage was also increased with HT exposure especially in PBW 343. The TaApe1L mRNA expression increased after priming in both the varieties after 4 h. But APE1 protein expression was higher in PBW 343, which can be correlated with DNA damage and repair. Lastly, it can be concluded that there is varietal difference in the HT sensitivity but 6 h exposure was detrimental to both the varieties. Also, drought priming improved HT tolerance by over expressing APE1.