A new type of immunomagnetic bead based on the metal-organic framework materials (MOFs) and the magnetic core (Fe3O4) was prepared for analysis of the mycotoxins in food samples. The MOF conjugated with the monoclonal antibodies (McAbs) was coated on the surface of Fe3O4 beads by reversed-phase microemulsion method, which could purify deoxynivalenol (DON), zearalenone (ZEN), T-2, and HT-2 mycotoxins at the same time. The composite (Fe3O4@AMP&ZnCl2@McAbs) was characterized by Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and Energy dispersive spectroscopy (EDS) mapping. The results showed that the synthesis of the composite was successful. The maximum toxin adsorption capacity per 100 mg of composite was DON 688.26 ng, ZEN 864.98 ng, and T-2/HT-2 2801.80 ng, and in adding recovery experiment, the recovery of four mycotoxins decreased slightly with the increase of usage times, but still maintained a high adsorption rate and stability. For effectiveness comparison and evaluation, the composite and commercial DZTMS-PREP immune affinity column were used to treat three samples of corn, wheat, and oat flour, and the purification effect of the two pretreatment methods on the four toxins was similar.
Keywords: Magnetic beads; Metal–organic framework materials; Mycotoxins.
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