Integrated genomic analysis using chromosomal microarray, fluorescence in situ hybridization and mate pair analyses: Characterization of a cryptic t(9;22)(p24.1;q11.2)/BCR-JAK2 in myeloid/lymphoid neoplasm with eosinophilia

Cancer Genet. 2020 Aug:246-247:44-47. doi: 10.1016/j.cancergen.2020.08.004. Epub 2020 Aug 7.

Abstract

The 2016 World Health Organization entity 'Myeloid/lymphoid neoplasms with eosinophilia and rearrangement of PDGFRA, PDGFRB or FGFR1, or with PCM1-JAK2' encompasses a group of rare neoplasms that result from the formation of a fusion gene that leads to expression of an aberrant tyrosine kinase. This entity also contains variant JAK2 fusion partners, and detection of this defining event can be facilitated by various cytogenetic and molecular methods. Cryptic rearrangements of 9p24/JAK2 can be particularly challenging to identify. We describe the use of chromosomal microarray analysis (CMA), fluorescence in situ hybridization (FISH) with a probe for JAK2, and genomic mate pair analysis to describe a complex karyotype with a t(9;22) that produced a functional BCR-JAK2 fusion, leading to the appropriate diagnosis for the patient. This case highlights the importance of using an integrated genomic approach to fully define complex aberrations to assign proper diagnoses.

Keywords: Acute leukemia; Chromosomal microarray analysis; Fluorescence in situ hybridization; Mate pair sequencing.

Publication types

  • Case Reports

MeSH terms

  • Chromosomes, Human, Pair 22 / genetics*
  • Chromosomes, Human, Pair 9 / genetics*
  • Eosinophilia / genetics
  • Eosinophilia / pathology*
  • Genomics / methods
  • Humans
  • In Situ Hybridization, Fluorescence / methods
  • Janus Kinase 2 / genetics*
  • Male
  • Microarray Analysis / methods
  • Middle Aged
  • Myeloproliferative Disorders / genetics
  • Myeloproliferative Disorders / pathology*
  • Prognosis
  • Proto-Oncogene Proteins c-bcr / genetics*
  • Translocation, Genetic*

Substances

  • JAK2 protein, human
  • Janus Kinase 2
  • BCR protein, human
  • Proto-Oncogene Proteins c-bcr

Supplementary concepts

  • Myeloproliferative Disorder, Chronic, with Eosinophilia