Nitrous oxide emissions in response to straw incorporation is regulated by historical fertilization

The incorporation of crop straw with fertilization is beneficial for soil carbon sequestration and cropland fertility improvement. Yet, relatively little is known about how fertilization regulates the emissions of the greenhouse gas nitrous oxide (N₂O) in response to straw incorporation, particularly in soils subjected to long-term fertilization regimes. Herein, the arable soil subjected to a 31-year history of five inorganic or organic fertilizer regimes (unfertilized; chemical fertilizer application, NPK; 200% NPK application, 2 × NPK; manure application, M; NPK plus manure application, NPKM) was incubated with and without rice straw to evaluate how historical fertilization influences the impact of straw addition on N₂O emissions. The results showed that compared to the unfertilized treatment, historical fertilization strongly increased N₂O emissions by 0.48- to 34-fold, resulting from increased contents of hot water-extracted organic carbon (HWEOC), NO₃^-, and available phosphorus (Olsen-P). Straw addition had little impact on N₂O emission from the unfertilized and NPK treatments, primarily due to Olsen-P limitation. In contrast, straw addition increased N₂O emissions by 102-316% from the 2 × NPK, M, and NPKM treatments as compared to the corresponding straw-unamended treatments. These results indicated that N₂O emissions in response to straw addition were largely regulated by historical fertilization. The N₂O emissions were closely associated with the depletion of NO₃^- and decoupled from change in NH₄⁺ content, suggesting that NO₃^- was the main substrate for N₂O production upon straw addition. The stoichiometric ratios of HWEOC to mineral N and mineral N to Olsen-P were key factors affecting N₂O emissions, underscoring the importance of resource stoichiometry in regulating N₂O emissions. In conclusion, historical fertilization largely regulated the impacts of crop straw incorporation on N₂O emissions via shifts in NO₃^- depletion and the stoichiometry of HWEOC, mineral N, and Olsen-P.