Multiple antibodied based immunoaffinity columns preparation for the simultaneous analysis of deoxynivalenol and T-2 toxin in cereals by liquid chromatography tandem mass spectrometry

Yanshen Li; Anqi Chen; Xin Mao; Mingxue Sun; Shupeng Yang; Juan Li; Yanli You; Yongning Wu; Guibin Jiang

doi:10.1016/j.foodchem.2020.127802

Multiple antibodied based immunoaffinity columns preparation for the simultaneous analysis of deoxynivalenol and T-2 toxin in cereals by liquid chromatography tandem mass spectrometry

Food Chem. 2021 Feb 1:337:127802. doi: 10.1016/j.foodchem.2020.127802. Epub 2020 Aug 9.

Authors

Yanshen Li¹, Anqi Chen², Xin Mao², Mingxue Sun², Shupeng Yang³, Juan Li⁴, Yanli You², Yongning Wu⁵, Guibin Jiang⁶

Affiliations

¹ College of Life Science, Yantai University, Yantai, Shandong 264005, PR China. Electronic address: liyanshen@ytu.edu.cn.
² College of Life Science, Yantai University, Yantai, Shandong 264005, PR China.
³ Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China.
⁴ Department of Chemical and Biochemical Engineering, University of Western Ontario, London, ON N6A5B9, Canada.
⁵ NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit (2019RU014), China National Center for Food Safety Risk Assessment, Beijing 100017, PR China.
⁶ State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.

PMID: 32795851
DOI: 10.1016/j.foodchem.2020.127802

Abstract

Deoxynivalenol (DON) and T-2 toxin are major trichothecenes contaminated in cereals, which might bring harmful effects to humans. In this research, mixed anti-DON and anti-T-2 mAb were used for multiple immunoaffinity columns (mIACs) preparation. Under the optimal conditions, column capacities were tested at 1280 ng/mL for DON and 1160 ng/mL for T-2 toxin. Regeneration investigation showed mIACs capacities were over 510 ng/mL for DON and 440 ng/mL for T-2 toxin in 10 recycle usages. Good performances were obtained when applying mIACs purification coupled UHPLC-MS/MS for spiked samples with limit of detection at 3-13 μg/kg and mean recoveries at 79.0-97.6%. Applying to estimate the exposure of DON and T-2 toxin in commercial samples, maize samples were 100% DON positive and rice samples were 40% DON positive while T-2 toxin was negative in all tested samples. The proposed method is reliable and suitable for monitoring DON and T-2 toxin in cereal samples.

Keywords: Cereals; Deoxynivalenol; Multiple immunoaffinity columns; T-2 Toxin; UHPLC-MS/MS.

MeSH terms

Chromatography, Affinity / methods*
Edible Grain / chemistry*
Food Contamination / analysis*
Limit of Detection
T-2 Toxin / analysis*
Tandem Mass Spectrometry / methods*
Trichothecenes / analysis*
Zea mays / chemistry

Substances

Trichothecenes
T-2 Toxin
deoxynivalenol