Strategies and perspectives to develop SARS-CoV-2 detection methods and diagnostics

Rekha Jalandra; Amit K Yadav; Damini Verma; Nishu Dalal; Minakshi Sharma; Rajeev Singh; Anil Kumar; Pratima R Solanki

doi:10.1016/j.biopha.2020.110446

Strategies and perspectives to develop SARS-CoV-2 detection methods and diagnostics

Biomed Pharmacother. 2020 Sep:129:110446. doi: 10.1016/j.biopha.2020.110446. Epub 2020 Jun 19.

Authors

Rekha Jalandra¹, Amit K Yadav², Damini Verma³, Nishu Dalal⁴, Minakshi Sharma⁵, Rajeev Singh⁶, Anil Kumar⁷, Pratima R Solanki⁸

Affiliations

¹ Gene Regulation Laboratory, National Institute of Immunology, New Delhi, 110067, India; Department of Zoology, Maharshi Dayanand University, Rohtak, 124001, India.
² Special Centre for Nanoscience, Jawaharlal Nehru University, New Delhi, 110067, India.
³ Amity Institute of Applied Sciences, Amity University, Uttar Pradesh, 201313, India.
⁴ Gene Regulation Laboratory, National Institute of Immunology, New Delhi, 110067, India; Department of Environmental Science, Satyawati College, Delhi University, New Delhi, 110052, India.
⁵ Department of Zoology, Maharshi Dayanand University, Rohtak, 124001, India.
⁶ Department of Environmental Science, Satyawati College, Delhi University, New Delhi, 110052, India.
⁷ Gene Regulation Laboratory, National Institute of Immunology, New Delhi, 110067, India. Electronic address: anilk@nii.ac.in.
⁸ Special Centre for Nanoscience, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: partima@mail.jnu.ac.in.

Abstract

To develop diagnostics and detection methods, current research is focussed on targeting the detection of coronavirus based on its RNA. Besides the RNA target, research reports are coming to develop diagnostics by targeting structure and other parts of coronavirus. PCR based detection system is widely used and various improvements in the PCR based detection system can be seen in the recent research reports. This review will discuss multiple detection methods for coronavirus for developing appropriate, reliable, and fast alternative techniques. Considering the current scenario of COVID-19 diagnostics around the world and an urgent need for the development of reliable and cheap diagnostic, various techniques based on CRISPR technology, antibody, MIP, LAMP, microarray, etc. should be discussed and tried.

Keywords: COVID-19; Coronavirus; Diagnosis; PCR.

Publication types

Review

MeSH terms

Animals
Betacoronavirus / isolation & purification*
COVID-19
COVID-19 Testing
Clinical Laboratory Techniques*
Coronavirus Infections / diagnosis*
Coronavirus Infections / virology
Humans
Pandemics
Pneumonia, Viral / diagnosis*
Pneumonia, Viral / virology
Polymerase Chain Reaction
RNA, Viral / isolation & purification
SARS-CoV-2
Sensitivity and Specificity

Substances

RNA, Viral