Genetically Encodable Fluorescent and Bioluminescent Biosensors Light Up Signaling Networks

Trends Biochem Sci. 2020 Oct;45(10):889-905. doi: 10.1016/j.tibs.2020.06.001. Epub 2020 Jul 10.

Abstract

Cell signaling networks are intricately regulated in time and space to determine the responses and fates of cells to different cues. Genetically encodable fluorescent and bioluminescent biosensors enable the direct visualization of these spatiotemporal signaling dynamics within the native biological context, and have therefore become powerful molecular tools whose unique benefits are being used to address challenging biological questions. We first review the basis of biosensor design and remark on recent technologies that are accelerating biosensor development. We then discuss a few of the latest advances in the development and application of genetically encodable fluorescent and bioluminescent biosensors that have led to scientific or technological breakthroughs.

Keywords: bioluminescence; compartmentalization; in vivo imaging; live-cell imaging; spatiotemporal regulation; super-resolution.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biosensing Techniques / methods
  • Luminescence*
  • Signal Transduction*