Influence of silver speciation on the inflammatory regulation of AgNPs anchoring onto titania nanotubes

Congling Yang; Ziquan Wang; Ke Huang; Jie Weng; Jianxin Wang; Jie Zhou; Bo Feng

doi:10.1016/j.colsurfb.2020.111199

Influence of silver speciation on the inflammatory regulation of AgNPs anchoring onto titania nanotubes

Colloids Surf B Biointerfaces. 2020 Oct:194:111199. doi: 10.1016/j.colsurfb.2020.111199. Epub 2020 Jun 16.

Authors

Congling Yang¹, Ziquan Wang², Ke Huang³, Jie Weng², Jianxin Wang², Jie Zhou⁴, Bo Feng⁵

Affiliations

¹ Key Laboratory of Advanced Technology for Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610066, China.
² Key Laboratory of Advanced Technology for Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
³ College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610066, China.
⁴ Key Laboratory of Advanced Technology for Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: zhoujie@home.swjtu.edu.cn.
⁵ Key Laboratory of Advanced Technology for Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: fengbo@swjtu.edu.cn.

PMID: 32585536
DOI: 10.1016/j.colsurfb.2020.111199

Abstract

AgNPs were immobilized on titania nanotubes (TNT) by chelation of polydopamine (PD) to generate a TNT/PD/AgNPs (TPAS) via a simple dipping method. The inflammatory regulation of the TPAS coating were investigated. To gain a deep insight into the transformation of AgNPs in macrophages, a cation exchange reaction was introduced for speciation analysis of AgNPs and Ag⁺ by inductively coupled plasma-mass spectrometry. Owing to the magic signal amplification strategy, the trace AgNPs and Ag⁺ in release media and even in macrophages were easily detected. In simulated inflammatory microenvironment, M1 macrophages promoted the cell-responsive release of Ag⁺ from TPAS at 3 d, which dampened inflammation. Then, macrophages reduced Ag⁺ by intracellular metabolites, leading to the formation of new AgNPs in cells. This study give a new sight for discovering the inflammatory regulation mechanism of silver containing biomaterials.

Keywords: Ag nanoparticles; Inflammatory response; Speciation analysis; Titania nanotubes.

MeSH terms

Metal Nanoparticles*
Nanotubes*
Silver
Titanium

Substances

titanium dioxide
Silver
Titanium