Genomic Analysis Reveals Antibiotic-Susceptible Clones and Emerging Resistance in Neisseria gonorrhoeae in Saskatchewan, Canada

Nidhi R Parmar; Reema Singh; Irene Martin; Sumudu R Perera; Walter Demczuk; Anthony Kusalik; Jessica Minion; Jo-Anne R Dillon

doi:10.1128/AAC.02514-19

Genomic Analysis Reveals Antibiotic-Susceptible Clones and Emerging Resistance in Neisseria gonorrhoeae in Saskatchewan, Canada

Antimicrob Agents Chemother. 2020 Aug 20;64(9):e02514-19. doi: 10.1128/AAC.02514-19. Print 2020 Aug 20.

Authors

Nidhi R Parmar^{1

2}, Reema Singh^{1

2}, Irene Martin³, Sumudu R Perera^{1

2}, Walter Demczuk³, Anthony Kusalik⁴, Jessica Minion⁵, Jo-Anne R Dillon^{6

2}

Affiliations

¹ Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
² Vaccine and Infectious Disease Organization-International Vaccine Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
³ Bacterial Pathogen Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
⁴ Department of Computer Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
⁵ Roy Romanow Provincial Laboratory, Regina, Saskatchewan, Canada.
⁶ Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada j.dillon@usask.ca.

Abstract

Whole-genome sequencing was used to identify mutations in antibiotic resistance-conferring genes to compare susceptibility predictions with MICs and to ascertain strain types in 99 isolates of Neisseria gonorrhoeae Genotypes associated with susceptibility, as well as MIC creep or emerging resistance, were noted. Phylogenomic analysis revealed three distinctive clades and putative gonococcal transmission linkages involving a tetracycline-resistant N. gonorrhoeae outbreak and the clonal spread of susceptible isolates in men.

Keywords: Neisseria gonorrhoeae; antimicrobial resistance prediction; single-nucleotide polymorphisms; strain types; transmission linkages; whole-genome sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / therapeutic use
Clone Cells
Drug Resistance, Bacterial / genetics
Genomics
Gonorrhea* / drug therapy
Gonorrhea* / epidemiology
Humans
Male
Microbial Sensitivity Tests
Neisseria gonorrhoeae* / genetics
Saskatchewan / epidemiology

Substances

Anti-Bacterial Agents

Grants and funding

148996/CIHR/Canada