Pretreatment of wheat (Triticum aestivum L.) seedlings with 2,4-D improves tolerance to salinity-induced oxidative stress and methylglyoxal toxicity by modulating ion homeostasis, antioxidant defenses, and glyoxalase systems

The commonly used herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) has an as yet undetermined protective role in mitigating salinity-induced damage in crop plants. The aim of this study was to explore the possible roles of antioxidant defense and methylglyoxal (MG) detoxification systems in enhancing salt tolerance in wheat (Triticum aestivum L. cv. Norin 61) seedlings following pretreatment with 2,4-D. Wheat seedlings were grown hydroponically, pretreated with 10 μM 2,4-D for 48 h, and then exposed to salt stress (150 and 250 mM NaCl) for the next five days. The protective effect of 2,4-D was associated with increased antioxidant enzyme activity and ascorbate and glutathione content, and with decreased malondialdehyde and hydrogen peroxide content and reduced electrolytic leakage. Application of 2,4-D increased glyoxalase enzyme activity, resulting in greater MG detoxification. Seedlings pretreated with 2,4-D showed improved growth, biomass, and leaf water content due to reductions in Na⁺ accumulation and increases in K⁺, Ca²⁺, and Mg²⁺ uptake. Overall, these results highlight the potential use of this common herbicide as a phytoprotectant against salinity stress.