Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC

Jiani Mai; Jie Liang; XianFu Liu; LiuPing Tan; Hui Xu; YaoHua Li; YuShan Zhou; ChuanChuan Yang; ChenXi Xin

doi:10.1155/2020/3950609

Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC

J Anal Methods Chem. 2020 Jan 29:2020:3950609. doi: 10.1155/2020/3950609. eCollection 2020.

Authors

Jiani Mai¹, Jie Liang^{1

2

3}, XianFu Liu⁴, LiuPing Tan¹, Hui Xu¹, YaoHua Li¹, YuShan Zhou¹, ChuanChuan Yang¹, ChenXi Xin¹

Affiliations

¹ College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, China.
² GuangXi Key Laboratory of Zhuang and Yao Ethnic Medicine, Nanning 530200, China.
³ Guangxi Zhuang Yao Medicine Center of Engineering and Technology, Nanning 530200, China.
⁴ Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, China.

Abstract

The pharmacodynamic effect of longan leaves was attributed to various components, especially the flavonoids. In this paper, a new strategy of quantitative analysis of multicomponents by a single marker (QAMS) method was first established to synchronously determine 5 components (ethyl gallate (C₁), astragalin (C₂), quercetin (C₃), luteolin (C₄), and kaempferol (C₅)) in Dimocarpus longan by ultra-performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC). Quercetin (C₃) was chosen as the internal reference. Relative correction factors (RCF_s, ƒ_s/i) of the other 4 components were calculated by two correction methods (multipoint correction and slope correction) to effectuate QAMS. At the same time, the difference between the results measured by the QAMS and external standard methods was compared to verify the accuracy of QAMS. Within the linear range, the results showed that all ƒ_s/i values were obtained with good durability under diverse chromatographic conditions (RSD < 2.28%). The quantitative results of 5 components in the leaves of Dimocarpus longan collected from 10 producing areas by different chromatographic systems and quantitative methods were significantly correlated (Pearson's r > 97.0%). The applicability and feasibility of the QAMS method established in this study were evaluated to be favorable for quality control of the leaves of Dimocarpus longan. As a new model of quality control, it can provide one more choice of multicomponent quality-control method in the absence of standard substances or instruments.