MRSA CC398 recovered from wild boar harboring new SCCmec type IV J3 variant

Margarida Sousa; Nuno Silva; Vítor Borges; João P Gomes; Luís Vieira; Manuela Caniça; Carmen Torres; Gilberto Igrejas; Patrícia Poeta

doi:10.1016/j.scitotenv.2020.137845

MRSA CC398 recovered from wild boar harboring new SCCmec type IV J3 variant

Sci Total Environ. 2020 Jun 20:722:137845. doi: 10.1016/j.scitotenv.2020.137845. Epub 2020 Mar 10.

Authors

Margarida Sousa¹, Nuno Silva², Vítor Borges³, João P Gomes³, Luís Vieira⁴, Manuela Caniça⁵, Carmen Torres⁶, Gilberto Igrejas⁷, Patrícia Poeta⁸

Affiliations

¹ MicroART - Microbiology and Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Veterinary and Animal Science Research Center (CECAV), UTAD, Vila Real, Portugal; Functional Genomics and Proteomics Unit, UTAD, Vila Real, Portugal; National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AR-HAI), National Institute of Health Dr. Ricardo Jorge (NIH), Lisbon, Portugal; Department of Food and Agriculture, Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.
² Moredun Research Institute (MRI), Pentlands Science Park, Bush Loan, Penicuik, Scotland, UK.
³ Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal.
⁴ Technology and Innovation Unit, Department of Human Genetics, National Institute of Health, Lisbon, Portugal.
⁵ National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AR-HAI), National Institute of Health Dr. Ricardo Jorge (NIH), Lisbon, Portugal; Centre for the Study of Animal Sciences (CECA/ICETA), University of Oporto, Oporto, Portugal.
⁶ Department of Food and Agriculture, Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.
⁷ Functional Genomics and Proteomics Unit, UTAD, Vila Real, Portugal; Department of Genetics and Biotechnology, UTAD, Vila Real, Portugal; Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, Caparica, Portugal.
⁸ MicroART - Microbiology and Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, Caparica, Portugal. Electronic address: ppoeta@utad.pt.

PMID: 32199375
DOI: 10.1016/j.scitotenv.2020.137845

Abstract

A methicillin-resistant Staphylococcus aureus CC398 was recovered from a wild female boar (Sus scrofa) in the north of Portugal, in 2013 (Sousa et al. 2017). Whole genome sequencing (WGS) revealed this strain carries a new variant of a mecA-containing staphylococcal chromosomal gene cassette (SCCmec) type IV with an uncommon J3 region. WGS studies can facilitate surveillance and provide more detailed characterization of bacterial clones circulating in the wild, reinforcing the need for a one health perspective to better understand and control antimicrobial resistance.

Keywords: Antimicrobial resistance; MRSA CC398; SCCmec IV; WGS; Wild animals.

MeSH terms

Animals
Anti-Bacterial Agents
Bacterial Proteins
Female
Methicillin-Resistant Staphylococcus aureus
Microbial Sensitivity Tests
Portugal
Staphylococcal Infections* / veterinary
Sus scrofa
Swine

Substances

Anti-Bacterial Agents
Bacterial Proteins