Purpose: The purpose of this study was to perform an in vitro evaluation of the protective effects of anticavity agents applied to enamel, by themselves and in combination with Er:YAG.
Materials and methods: In this study 150 extracted third molars were used. Measurements were made using DIAGNOdent, and samples with scores of 0-13 were included in the study. These were divided into 15 groups (n = 20). Use of the agents sodium fluoride (NaF), tricalcium phosphate (Ca3PO4), titanium tetrafluoride (TiF4), Tooth Mousse (CPP-ACP), MI Paste Plus (CPP-ACP), and NovaMin (bioactive glass), individually and then in combination with Er:YAG laser, was assessed based on their effects on microhardness values. After treatment, the groups were exposed to a demineralisation solution. Statistical analyses were conducted using SPSS 20.0 package software.
Results: The highest test result value was observed in the TFL (TiF4/Er:YAG laser) group. Statistically significant differences were determined among all the groups (p <0.05). When the groups in which the agents were applied alone were compared with those involving combined use of Er:YAG laser, combined use provided significantly higher microhardness values, with the exception of the TML group (Tooth Mousse/Er:YAG laser) (p <0.05).
Conclusion: Within the limits of this study, the combined use of remineralisation agents and Er:YAG laser elicited better results than using the agents alone. The most effective remineralisation agent was TiF4/Er:YAG laser, which may be considered an alternative method for protecting the enamel against demineralisation.
Keywords: Er:YAG laser; bioactive glass; casein phosphopeptide amorphous calcium phosphate; demineralisation; remineralisation; titanium tetrafluoride.