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J Chromatogr. 1988 Jul 8;445(1):199-206.

Rapid separation of flavonoids by analytical high-speed counter-current chromatography.

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  • 1Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.


A commercial model of the analytical high-speed counter-current chromatography instrument was used for separation of flavonoids from a crude ethanol extract of dried fruits of sea buckthorn (Hippophae rhamnoides). Using a two-phase solvent system of chloroform-methanol-water (4:3:2), a five-fold increase in flow-rate of the mobile phase from 60 to 300 ml/h resulted in a rapid separation of five components in less than 15 min without significant loss in peak resolution. Major flavonoid component, isorhamnetin, was identified in its pure state by mass spectrometric analysis.

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