Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry

Ileana S Mauldin; Natasha D Sheybani; Samuel J Young; Richard J Price; Craig L Slingluff

doi:10.1016/bs.mie.2019.05.038

Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry

Methods Enzymol. 2020:635:81-93. doi: 10.1016/bs.mie.2019.05.038. Epub 2019 Jun 29.

Authors

Ileana S Mauldin¹, Natasha D Sheybani², Samuel J Young¹, Richard J Price², Craig L Slingluff³

Affiliations

¹ Department of Surgery, University of Virginia, Charlottesville, VA, United States.
² Department of Biomedical Engineering; University of Virginia, Charlottesville, VA, United States.
³ Department of Surgery, University of Virginia, Charlottesville, VA, United States. Electronic address: cls8h@virginia.edu.

Abstract

In a variety of solid tumors, the presence of higher densities of tumor-infiltrating lymphocytes or tertiary lymphoid structures (TLS) are correlated with prolonged patient survival. Murine studies are usually required to define mechanisms that govern immunologic infiltrate in tumors. However, few methods have been described that could enable a more comprehensive understanding of the functionality of intratumoral immune infiltrate and TLS in solid murine cancers. In this chapter, we describe multiplex immunohistochemistry and microscopy approaches for identifying, characterizing, and quantifying intratumoral immune infiltrate and TLS in murine tumor models.

Keywords: Glioblastoma; IHC; Immunology; Melanoma; Multiplex immunohistochemistry; Tertiary lymphoid structures; Tumor infiltrating lymphocytes.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Humans
Immunohistochemistry
Lymphocytes, Tumor-Infiltrating
Mice
Neoplasms*
Tertiary Lymphoid Structures*
Tumor Microenvironment

Abstract

Publication types

MeSH terms

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