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    Biochem Biophys Res Commun. 1988 Dec 30;157(3):1309-15.

    Nucleotide sequence and tissue distribution of the human mitochondrial aspartate aminotransferase mRNA.

    Pol S, Bousquet-Lemercier B, Pave-Preux M, Pawlak A, Nalpas B, Berthelot P, Hanoune J, Barouki R.

    Source

    Inserm U-99, Hôpital Henri Mondor, Creteil, France.

    Abstract

    The cDNA of human mitochondrial aspartate aminotransferase (E.C.2.6.1.1.) was isolated from a human liver cDNA library using a rat mitochondrial aspartate aminotransferase cDNA as probe. The sequence of this cDNA gives a predicted aminoacid sequence for the human presequence and for the human mature protein exhibiting respectively 93% and 95% homology with rat sequences. A Northern blot of total RNA, isolated from various human tissues and hybridized with this cDNA, revealed a single 2.4 Kb RNA band. Mitochondrial aspartate aminotransferase RNA was clearly detected in human kidney, placenta, stomach and spleen as well as in both fetal and adult liver.

    PMID:
    3207426
    [PubMed - indexed for MEDLINE]

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