Dendritic cells transduced with glioma-expressed antigen 2 recombinant adenovirus induces specific cytotoxic lymphocyte response and anti-tumor effect in mice

Gaohai Shao; Changlong Zhou; Kunlong Ma; Wang Zhao; Guibo Feng; Qijiang Xiong; Ling Yang; Zhao Yang

doi:10.1186/s12950-020-0239-6

Dendritic cells transduced with glioma-expressed antigen 2 recombinant adenovirus induces specific cytotoxic lymphocyte response and anti-tumor effect in mice

J Inflamm (Lond). 2020 Jan 31:17:3. doi: 10.1186/s12950-020-0239-6. eCollection 2020.

Authors

Gaohai Shao¹, Changlong Zhou², Kunlong Ma¹, Wang Zhao², Guibo Feng², Qijiang Xiong², Ling Yang², Zhao Yang²

Affiliations

¹ 1Department of orthopedics, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160 China.
² 2Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160 China.

Abstract

Introduction: Glioma is an aggressive common cancer with high mortality worldwide. Up to date, the effective medical therapeutical strategy is limited. Numerous previous studies have indicated that glioma-expressed antigen 2 (GLEA2) might be an attractive prognostic glioma biomarker.

Methods: In this experiment, dendritic cells (DCs) transduced with GLEA2 recombinant adenovirus were utilized to generate cytotoxic lymphocytes (CTLs) in vitro. Additionally, trimera mice were immunized with the transduced DCs to generate CTLs in vivo.

Results: The data demonstrated that GLEA2 transduced DCs could effectively generate specific CTL response against glioma without lysing autologous lymphocytes. Moreover, GLEA2 transduced DCs significantly attenuated the tumor growth and prolonged the life span of tumor bearing mice.

Conclusions: These findings suggested that DCs transduced with GLEA2 recombinant adenovirus could generate effective CTL mediated anti-tumor response, and might represent insight in glioma therapy.

Keywords: Anti-tumor; CTL; Dendritic cells; GLEA2.