Generation and characterization of a human iPSC line derived from congenital clubfoot amniotic fluid cells

Jing Wang; Yazhou Cui; Kaixuan Xing; Jing Luan; Jinxiang Han

doi:10.1016/j.scr.2020.101712

Generation and characterization of a human iPSC line derived from congenital clubfoot amniotic fluid cells

Stem Cell Res. 2020 Mar:43:101712. doi: 10.1016/j.scr.2020.101712. Epub 2020 Jan 17.

Authors

Jing Wang¹, Yazhou Cui², Kaixuan Xing², Jing Luan², Jinxiang Han³

Affiliations

¹ School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Ji'nan, Shandong, China; Shandong Medicinal Biotechnology Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan, Shandong, China; Key Laboratory for Biotech-Drugs of National Health Commission, Ji'nan, Shandong, China.
² Shandong Medicinal Biotechnology Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan, Shandong, China; Key Laboratory for Biotech-Drugs of National Health Commission, Ji'nan, Shandong, China.
³ School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Ji'nan, Shandong, China; Shandong Medicinal Biotechnology Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan, Shandong, China; Key Laboratory for Biotech-Drugs of National Health Commission, Ji'nan, Shandong, China. Electronic address: jxhan9888@aliyun.com.

PMID: 31986484
DOI: 10.1016/j.scr.2020.101712

Abstract

A human induced pluripotent stem cell (iPSC) line (SMBCi002-A) was established from amniocytes (amniotic fluid cells, AFDCs) from a 16-week-old female fetus with clubfoot. Reprogramming and enhancing factors OCT4, SOX2, KLF4, MYC, LIN28 and EBNA1 were delivered using a nonintegrative strategy based on episomal plasmids. Validation assays indicated that this iPSC line has full pluripotency, differentiation potential and genetic stability. Currently, the etiology and mechanism of clubfoot remain unclear; therefore, this in vitro cellular model would provide a useful resource for understanding the pathology of this disease.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Amniotic Fluid / metabolism*
Cells, Cultured
Clubfoot / genetics*
Female
Fetus
Humans
Induced Pluripotent Stem Cells / metabolism*
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