Science. 1977 Jan 28;195(4276):393-4.

Plasmid detection and sizing in single colony lysates.

Abstract

A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.

PMID:: 318764; [PubMed - indexed for MEDLINE]

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