The CRISPR/Cas system is currently widely used for genome editing. The procedure of genome editing includes two necessary steps: (i) searching for the most effective guide RNA, and (ii) analyzing clones for presence of the desired mutation. This review presents the methods used to assess the efficiency of the CRISPR/Cas system and to confirm mutation in the target locus and discusses their advantages and disadvantages. It aims to provide information that could help researchers to choose a technique most appropriate for their specific tasks and available resources.
Keywords: CRISPR/Сas9; DHPLC; ENIT; HRMA; IDAA; LDR; NGS; RFLP; SNP; SSCP; SURVEYOR; T7E1; TIDE; cbPCR; droplet digital PCR; pDR/FACS.