Hospital clones of Panton-Valentine leukocidin-positive and methicillin-resistant Staphylococcus aureus circulating in the Tehran community

Objectives: The emergence of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus has become a serious challenge in hospital and community settings. Therefore, this study was designed to determine the molecular characteristics and genetic relationship of PVL-positive S. aureus.

Methods: A total of 116 S. aureus isolates were collected from outpatients and inpatients within 48 h after admission. For PVL-positive strains, antibiogram testing, toxin gene profile, agr grouping, SCCmec typing and genotyping by pulsed-field gel electrophoresis (PFGE) were performed.

Results: Of 116 isolates, 13 (11.2%) harboured the PVL gene, 5 isolates (38.4%) were methicillin-resistant S. aureus (MRSA) with agr group I and SCCmec III. Of these, four strains were hospital-associated MRSA (HA-MRSA) that moved to the community and one strain was atypical community-associated MRSA (CA-MRSA), with characteristics of being PVL-positive, susceptible to all tested antibiotics, including clindamycin, with SCCmec III. This was recovered from a burn patient less than 24 h after the patient's admission and fulfilled the Centers for Disease Control and Prevention (CDC) criteria for CA-MRSA, except for the type of SCCmec. High diversity was found among PVL-positive strains by PFGE. All PVL gene-positive strains had hly and psms genes, but the tsst-1 gene was found just in methicillin-susceptible S. aureus (MSSA).

Conclusion: The results indicate that the distribution of the PVL gene in MSSA isolates was higher than in MRSA strains, and various PVL-positive resistant and virulent clones of HA-MRSA are circulating in the community. Therefore, implementing systematic surveillance is necessary for the evaluation of common PVL-positive S. aureus clones in the community.