Current advances in engineering tools for Pichia pastoris

Jasmin E Fischer; Anton Glieder

doi:10.1016/j.copbio.2019.06.002

Current advances in engineering tools for Pichia pastoris

Curr Opin Biotechnol. 2019 Oct:59:175-181. doi: 10.1016/j.copbio.2019.06.002. Epub 2019 Aug 27.

Authors

Jasmin E Fischer¹, Anton Glieder²

Affiliations

¹ Bisy e.U., Hofstätten/Raab, Austria; Institute of Molecular Biotechnology, NAWI Graz University of Technology, Graz, Austria.
² Bisy e.U., Hofstätten/Raab, Austria; Institute of Molecular Biotechnology, NAWI Graz University of Technology, Graz, Austria. Electronic address: anton.glieder@tugraz.at.

PMID: 31470258
DOI: 10.1016/j.copbio.2019.06.002

Abstract

In the past two to five years innovative DNA tools and inventive methodologies accelerated the speed of engineering of Komagataella phaffii (Pichia pastoris) for the efficient expression of intracellular and secreted proteins. Going beyond the standard approaches employing single heterologous genes or simultaneous expression of several different genes under the control of identical promoter and terminator sequences, balanced and consecutive co-expression of multiple genes (a, b) combined with simple host genome editing (c) now opens new opportunities for manufacturing of recombinant proteins or chemicals made by whole cell biocatalysis or synthetic biology.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Gene Editing*
Pichia*
Promoter Regions, Genetic
Recombinant Proteins
Synthetic Biology

Substances

Recombinant Proteins