Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Immunol. 1988 Oct;25(10):1011-7.

Immunochemical studies on pneumococcal type 37 capsular polysaccharide.

Author information

  • 1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, NY 14642.

Abstract

Pneumococcal type 37 capsular polysaccharide was obtained free of contaminants by affinity chromatography on Con-A, wheat germ agglutinin, Maclura pomifera lectin and HOPC-8 mouse myeloma protein affinity columns. The immunochemical reactivity of native and periodate oxidized borohydride reduced type 37 polysaccharide antigen with polyclonal rabbit and monoclonal mouse anti-Pn37 hybridoma antibodies was studied by quantitative precipitation. Quantitative hapten inhibition studies, employing the isomeric series of alpha- and beta-(1----2), (1----3), (1----4) and (1----6)-linked glucobioses as competitive inhibitors of antibody precipitation establish a specificity for anti-Pn37 antibody directed at least in part, against the Glc beta(1----2) Glc (sophorosyl) unit. A high mol. wt, D-glucose containing polysaccharide antigen, cross-reactive with rabbit anti-Pn37 is reported which was found to occur in the culture medium of 7 of 19 strains of Actinomyces examined.

PMID:
3146022
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk