Fusion of pseudorabies virus glycoproteins to IgG Fc enhances protective immunity against pseudorabies virus

Jianglong Li; Xiangmin Li; Genxi Hao; Huawei Zhang; Huiling Yang; Huanchun Chen; Ping Qian

doi:10.1016/j.virol.2019.07.027

Fusion of pseudorabies virus glycoproteins to IgG Fc enhances protective immunity against pseudorabies virus

Virology. 2019 Oct:536:49-57. doi: 10.1016/j.virol.2019.07.027. Epub 2019 Jul 30.

Authors

Jianglong Li¹, Xiangmin Li², Genxi Hao¹, Huawei Zhang¹, Huiling Yang¹, Huanchun Chen², Ping Qian³

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
² State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China; Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
³ State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China; Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. Electronic address: qianp@mail.hzau.edu.cn.

PMID: 31400549
DOI: 10.1016/j.virol.2019.07.027

Abstract

Molecular adjuvants are vaccine delivery vehicle to increase specific antigens effectiveness. Herein, we concentrated on IgG Fc, an effective molecular adjuvant, to develop novel pseudorabies virus (PRV) subunit vaccines. Two major protective antigen genes of PRV were constructed and linked into the mouse IgG Fc fragment. The gD, gD-IgG2aFc, gB and gB-IgG2aFc proteins were expressed using a baculovirus system. Mice intranasally immunized with gD-IgG2aFc or gB-IgG2aFc subunit vaccine exhibited significantly higher PRV-specific antibodies, neutralizing antibodies and intracellular cytokines than the mice intranasally immunized with gD or gB subunit vaccine. Moreover, no histopathological lesions were observed in mice immunized with gB-IgG2aFc subunit vaccine via histopathology examination. Further, the gB-IgG2aFc subunit vaccine was efficient for PRV infection compared with live attenuated vaccine. Overall, these results suggest that IgG2a Fc fragment, as a potential molecular adjuvant, fused with PRV antigen might be a promising and efficient PRV vaccine candidate.

Keywords: IgG Fc; Molecular adjuvants; Pseudorabies virus; Subunit vaccine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / administration & dosage
Adjuvants, Immunologic / biosynthesis
Adjuvants, Immunologic / genetics
Animals
Antibodies, Viral / biosynthesis
Baculoviridae / genetics
Baculoviridae / metabolism
Cytokines / genetics
Cytokines / immunology
Epithelial Cells / pathology
Epithelial Cells / virology
Female
Glycoproteins / administration & dosage
Glycoproteins / biosynthesis
Glycoproteins / genetics
Herpesvirus 1, Suid / drug effects*
Herpesvirus 1, Suid / genetics
Herpesvirus 1, Suid / immunology
Herpesvirus 1, Suid / pathogenicity
Immunization
Immunoglobulin Fc Fragments / administration & dosage
Immunoglobulin Fc Fragments / biosynthesis*
Immunoglobulin Fc Fragments / genetics
Immunoglobulin G / administration & dosage
Immunoglobulin G / biosynthesis
Immunoglobulin G / genetics
Kidney / pathology
Kidney / virology
Mice
Mice, Inbred BALB C
Pseudorabies / immunology
Pseudorabies / mortality
Pseudorabies / prevention & control*
Pseudorabies / virology
Pseudorabies Vaccines / administration & dosage
Pseudorabies Vaccines / biosynthesis*
Pseudorabies Vaccines / genetics
Recombinant Fusion Proteins / administration & dosage
Recombinant Fusion Proteins / biosynthesis*
Recombinant Fusion Proteins / genetics
Survival Analysis
Swine
Vaccines, Subunit
Viral Envelope Proteins / administration & dosage
Viral Envelope Proteins / biosynthesis*
Viral Envelope Proteins / genetics

Substances

Adjuvants, Immunologic
Antibodies, Viral
Cytokines
Glycoproteins
Immunoglobulin Fc Fragments
Immunoglobulin G
Pseudorabies Vaccines
Recombinant Fusion Proteins
Vaccines, Subunit
Viral Envelope Proteins