Identification of a Potential Inhibitor Targeting MurC Ligase of the Drug Resistant Pseudomonas aeruginosa Strain through Structure-Based Virtual Screening Approach and In Vitro Assay

Curr Pharm Biotechnol. 2019;20(14):1203-1212. doi: 10.2174/1389201020666190719123133.

Abstract

Background & objective: Pseudomonas aeruginosa shows resistance to a large number of antibiotics, including carbapenems and third generation cephalosporin. According to the World Health Organization global report published in February 2017, Pseudomonas aeruginosa is on the priority list among resistant bacteria, for which new antibiotics are urgently needed. Peptidoglycan serves as a good target for the discovery of novel antimicrobial drugs.

Methods: Biosynthesis of peptidoglycan is a multi-step process involving four mur enzymes. Among these enzymes, UDP-N-acetylmuramate-L-alanine ligase (MurC) is considered to be an excellent target for the design of new classes of antimicrobial inhibitors in gram-negative bacteria.

Results: In this study, a homology model of Pseudomonas aeruginosa MurC ligase was generated and used for virtual screening of chemical compounds from the ZINC Database. The best screened inhibitor i.e. N, N-dimethyl-2-oxo-2,3-dihydro-1H-1,3-benzodiazole-5-sulfonamide was then validated experimentally through inhibition assay.

Conclusion: The presented results based on combined computational and in vitro analysis open up new horizons for the development of novel antimicrobials against this pathogen.

Keywords: MurC ligase; P. aeruginosa; molecular docking; peptidoglycan; structure modeling; virtual screening..

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Drug Discovery / methods*
  • Drug Resistance, Bacterial / drug effects*
  • Drug Resistance, Bacterial / genetics
  • Escherichia coli / drug effects
  • High-Throughput Screening Assays
  • Humans
  • Molecular Dynamics Simulation*
  • Peptide Synthases / antagonists & inhibitors*
  • Peptide Synthases / genetics
  • Peptidoglycan / metabolism
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / enzymology
  • Structure-Activity Relationship

Substances

  • Anti-Bacterial Agents
  • Peptidoglycan
  • Peptide Synthases
  • UDP-N-acetylmuramoyl-alanine synthetase