Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides

Hua Li; Wei Dong; Yong Liu; Haiyan Zhang; Gang Wang

doi:10.3389/fmicb.2019.01254

Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides

Front Microbiol. 2019 Jun 26:10:1254. doi: 10.3389/fmicb.2019.01254. eCollection 2019.

Authors

Hua Li^{1

2}, Wei Dong², Yong Liu³, Haiyan Zhang^{1

2}, Gang Wang²

Affiliations

¹ Institute of Microbial Engineering, Henan University, Kaifeng, China.
² School of Life Sciencel, Henan University, Kaifeng, China.
³ College of Chemistry and Chemical Engineering, Henan University, Kaifeng, China.

Abstract

Three nickel-binding peptides were screened from a phage peptide library and displayed separately or in combination with surface-engineered Saccharomyces cerevisiae EBY100. The sorption of nickel ions on the surface of yeast cells increased with the increasing number of nickel Ni(II)-binding peptides displayed. The combined expression of the three peptides by EBY100/pYD1-N123 demonstrated the highest sorption of Ni(II) (2.603 ± 0.004 g g^-1, dry weight) and an enhanced sorption capacity of 60.15%, compared to S. cerevisiae EBY100. An orthogonal test for yeast immobilization was designed. A maximum sorption capability of 68.62% was observed for a treatment at 25°C with 2.0% calcium chloride and 3.0% sodium alginate.

Keywords: biosorption; immobilization; nickel-binding peptide; phage peptide library; surface-engineered yeast.