Delivery of siRNA therapeutics using cowpea chlorotic mottle virus-like particles

Biomater Sci. 2019 Aug 1;7(8):3138-3142. doi: 10.1039/c9bm00785g. Epub 2019 Jul 1.

Abstract

While highly promising in medicine, gene therapy requires delivery agents to protect and target nucleic acid therapeutics. We developed a plant viral siRNA delivery platform making use of self-assembling cowpea chlorotic mottle virus (CCMV). CCMV was loaded with siRNAs targeting GFP or FOXA1; to further enhance cell uptake and intracellular trafficking, resulting in more efficient gene knockdown, we appended CCMV with a cell penetrating peptide (CPP), specifically M-lycotoxin peptide L17E.

MeSH terms

  • Bromovirus / metabolism*
  • Cell-Penetrating Peptides / metabolism
  • Drug Carriers / metabolism*
  • Gene Silencing
  • Genetic Therapy*
  • HeLa Cells
  • Hepatocyte Nuclear Factor 3-alpha / deficiency
  • Hepatocyte Nuclear Factor 3-alpha / genetics
  • Humans
  • MCF-7 Cells
  • RNA, Small Interfering / genetics*
  • RNA, Small Interfering / metabolism*

Substances

  • Cell-Penetrating Peptides
  • Drug Carriers
  • FOXA1 protein, human
  • Hepatocyte Nuclear Factor 3-alpha
  • RNA, Small Interfering