BacMam System for Rapid Recombinant Protein Expression in Mammalian Cells

Methods Mol Biol. 2020:2125:205-208. doi: 10.1007/7651_2019_249.

Abstract

Baculovirus expression vector system (BEVS) is an established technology for recombinant protein expression in insect cells. Further, BEVS-mediated gene transduction of mammalian cells (BacMam) is emerging as a technique for high level recombinant protein expression in mammalian cells. Here, we describe generic method in using BEVS as a BacMam for rapid recombinant protein expression in mammalian cells.

Keywords: BEVS; Bac-to-Bac; BacMam.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Baculoviridae / metabolism*
  • Gene Expression*
  • HEK293 Cells
  • Humans
  • Recombinant Proteins / metabolism*
  • Transfection / methods*

Substances

  • Recombinant Proteins