Evidence that the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate is a product of the methylreductase reaction in Methanobacterium

Biochem Biophys Res Commun. 1987 Dec 16;149(2):455-60. doi: 10.1016/0006-291x(87)90389-5.

Abstract

When 7-mercaptoheptanoylthreonine phosphate (HS-HTP) was used as the sole source of electrons for reductive demethylation of 2-(methylthio)-ethanesulfonic acid (CH3-S-CoM) by cell extracts of Methanobacterium thermoautotrophicum strain delta H, the heterodisulfide of coenzyme M and HS-HTP (CoM-S-S-HTP) was quantitatively produced: HS-HTP + CH3-S-CoM----CH4 + CoM-S-S-HTP. CH4 and CoM-S-S-HTP were produced stoichiometrically in a ratio of 1:1. Coenzyme M (HS-CoM) inhibited HS-HTP driven methanogenesis indicating that CH3-S-CoM rather than HS-CoM was the substrate for CoM-S-S-HTP formation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Euryarchaeota / metabolism*
  • Mercaptoethanol / analogs & derivatives*
  • Mesna / metabolism*
  • Methane / metabolism
  • Oxidation-Reduction
  • Phosphothreonine / analogs & derivatives*
  • Phosphothreonine / metabolism
  • Sulfides / metabolism
  • Threonine / analogs & derivatives*

Substances

  • Sulfides
  • 7-mercaptoheptanoylthreonine phosphate
  • Phosphothreonine
  • Threonine
  • Mercaptoethanol
  • Mesna
  • Methane