EMBO J. 1987 Oct;6(10):2891-6.

Tyrosine sulfation, a post-translational modification of microvillar enzymes in the small intestinal enterocyte.

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Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark.

Abstract

Protein sulfation in small intestinal epithelial cells was studied by labelling of organ cultured mucosal explants with [35S]-sulfate. Six bands in SDS-PAGE became selectively labelled; four, of 250, 200, 166 and 130 kd, were membrane-bound and two, of 75 and 60 kd, were soluble. The sulfated membrane-bound components were all enriched in the microvillar fraction but either absent or barely detectable in intracellular or basolateral membranes. Immunopurification of sucrase-isomaltase, maltase-glucoamylase, aminopeptidase N and aminopeptidase A showed that these microvillar enzymes become sulfated. Most if not all the sulfate was bound to tyrosine residues rather than to the carbohydrate of the microvillar enzymes, showing that this type of modification can occur on plasma membrane proteins as well as on secretory proteins.

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PMCID:: PMC553723

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Biosynthesis of intestinal microvillar proteins. The intracellular transport of aminopeptidase N and sucrase-isomaltase occurs at different rates pre-Golgi but at the same rate post-Golgi. [FEBS Lett. 1985]
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Cited by 3 PubMed Central articles

Predicting sulfotyrosine sites using the random forest algorithm with significantly improved prediction accuracy. [BMC Bioinformatics. 2009]
Predicting sulfotyrosine sites using the random forest algorithm with significantly improved prediction accuracy.
Yang ZR. BMC Bioinformatics. 2009 Oct 29; 10:361. Epub 2009 Oct 29.
Sulfation, the up-and-coming post-translational modification: its role and mechanism in protein-protein interaction. [J Proteome Res. 2007]
Sulfation, the up-and-coming post-translational modification: its role and mechanism in protein-protein interaction.
Woods AS, Wang HY, Jackson SN. J Proteome Res. 2007 Mar; 6(3):1176-82. Epub 2007 Jan 26.
Tyrosine sulphation is not required for microvillar expression of intestinal aminopeptidase N. [Biochem J. 1988]
Tyrosine sulphation is not required for microvillar expression of intestinal aminopeptidase N.
Danielsen EM. Biochem J. 1988 Aug 15; 254(1):219-22.

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