Objective: To study the significance of dishevelled (DVL) proteins in the Wnt signaling pathway in the pathogenesis and prognosis of childhood acute lymphoblastic leukemia (ALL).
Methods: A total of 33 children with new-onset ALL were enrolled as the case group. According to the degree of risk, they were divided into 3 groups: low-risk (n=14), intermediate-risk (n=5) and high-risk (n=14). A total of 29 children with immune thrombocytopenia were enrolled as the control group. At diagnosis and on day 33 of induction therapy, 2 mL bone marrow samples were collected from the case and control groups, and qRT-PCR was used to measure the mRNA expression of DVL1, DVL2 and DVL3 in blood cells of bone marrow.
Results: The mRNA expression of DVL1, DVL2 and DVL3 in the case group in the incipient stage was significantly higher than that in the remission stage and the control group (P<0.05). Compared with the control group, the case group had a significant increase in the mRNA expression of DVL2 in the remission stage (P<0.05). The mRNA expression of DVL2 was significantly higher than that of DVL1 and DVL3 in both remission and incipient stages (P<0.05). The high- and intermediate-risk groups had significantly higher mRNA expression of DVL1 and DVL2 than the low-risk group (P<0.05). The mRNA expression of DVL2 was significantly higher than that of DVL1 and DVL3 in the low-, intermediate- and high-risk groups (P<0.05).
Conclusions: The change in the expression of DVL, especially DVL2, in the Wnt signal pathway has certain significance in the pathogenesis and prognosis of childhood ALL.
目的: 探讨Wnt信号通路中散乱蛋白（DVL）对儿童急性淋巴细胞白血病（ALL）发病及预后的意义。
方法: 选取33例新发ALL患儿为病例组，根据危险度将ALL患儿分为低危组（n=14）、中危组（n=5）和高危组（n=14）；29例免疫性血小板减少症（ITP）患儿为对照组。采集病例组患儿初发及诱导治疗第33天时，以及对照组骨髓血各2 mL，采用实时荧光定量聚合酶链反应（qRT-PCR）检测骨髓血细胞DVL1、DVL2、DVL3 mRNA表达。
结果: 病例组初发期DVL1、DVL2、DVL3 mRNA表达水平高于缓解期及对照组（P < 0.05），与对照组比较，病例组缓解期DVL2 mRNA水平升高（P < 0.05）。DVL2 mRNA表达水平在病例组初发期及缓解期均高于DVL 1、DVL 3 mRNA（P < 0.05）。高、中危组DVL1、DVL2 mRNA表达水平高于低危组（P < 0.05）。DVL2 mRNA表达水平在低、中、高危组均高于DVL1、DVL3 mRNA（P < 0.05）。
结论: Wnt通路中DVL，尤其是DVL2的表达变化可能对儿童ALL的发病及预后有一定意义。