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Purification and partial characterization of lysosomal neuraminidase from human placenta.
Lysosomal neuraminidase and beta-galactosidase are present in a complex together with a 32-kDa protective protein. This complex has been purified and the different components have been dissociated using potassium isothiocyanate (KSCN) treatment. beta-Galactosidase remains catalytically active, but neuraminidase loses its activity upon dissociation. The inactive dissociated neuraminidase was purified by removing the remaining non-dissociated beta-galactosidase/protective protein complex using beta-galactosidase-specific affinity chromatography. The dissociated neuraminidase material shows two major polypeptides on SDS-PAGE with an apparent molecular mass of 76 kDa and 66 kDa. Subsequently the 32-kDa protective protein was dissociated from the beta-galactosidase/protective protein complex, and purified. Antibodies raised against the dissociated inactive neuraminidase preparation specifically immunoprecipitate the active neuraminidase present in the complex with beta-galactosidase and protective protein. By immunoblotting evidence is provided that the 76-kDa protein is a subunit of neuraminidase which, in association with the 32-kDa protective protein, is essential for neuraminidase activity.
PMID: 3102233 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Molecular mechanism of lysosomal sialidase deficiency in galactosialidosis involves its rapid degradation.
Vinogradova MV, Michaud L, Mezentsev AV, Lukong KE, El-Alfy M, Morales CR, Potier M, Pshezhetsky AV.
Biochem J. 1998 Mar 1; 330 ( Pt 2):641-50.
[Biochem J. 1998]
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Ganglioside GM3 sialidase activity in fibroblasts of normal individuals and of patients with sialidosis and mucolipidosis IV. Subcellular distribution and and some properties.
Lieser M, Harms E, Kern H, Bach G, Cantz M.
Biochem J. 1989 May 15; 260(1):69-74.
[Biochem J. 1989]
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Human placental beta-galactosidase. Characterization of the dimer and complex forms of the enzyme.
Hubbes M, D'Agrosa RM, Callahan JW.
Biochem J. 1992 Aug 1; 285 ( Pt 3):827-31.
[Biochem J. 1992]
Patient Drug Information
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Potassium (Glu-K® , K+ 10® , K+ 8® , ...)
Potassium is essential for the proper functioning of the heart, kidneys, muscles, nerves, and digestive system. Usually the food you eat supplies all of the potassium you need. However, certain diseases (e.g., kidney dis...