Isolation and oncogenic potential of a novel human src-like gene

Mol Cell Biol. 1986 Dec;6(12):4195-201. doi: 10.1128/mcb.6.12.4195-4201.1986.

Abstract

We have isolated cDNA molecules representing the complete coding sequence of a new human gene which is a member of the src family of oncogenes. Nucleotide sequence analysis revealed that this gene, termed slk, encoded a 537-residue protein which was 86% identical to the chicken proto-oncogene product, p60c-src, over a stretch of 191 amino acids at its carboxy terminus. In contrast, only 6% amino acid homology was observed within the amino-terminal 82 amino acid residues of these two proteins. It was possible to activate slk as a transforming gene by substituting approximately two-thirds of the slk coding sequence for an analogous region of the v-fgr onc gene present in Gardner-Rasheed feline sarcoma virus. The resulting hybrid protein molecule expressed in transformed cells demonstrated protein kinase activity with specificity for tyrosine residues.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cells, Cultured
  • DNA / analysis
  • Endothelium
  • Genes*
  • Humans
  • Mice
  • Oncogene Protein pp60(v-src)
  • Oncogenes*
  • Proto-Oncogene Mas
  • Retroviridae Proteins / genetics*

Substances

  • MAS1 protein, human
  • Proto-Oncogene Mas
  • Retroviridae Proteins
  • DNA
  • Oncogene Protein pp60(v-src)

Associated data

  • GENBANK/M14676