P-Glycoprotein Antibody Decorated Porous Hydrogel Particles for Capture and Release of Drug-Resistant Tumor Cells

Adv Healthc Mater. 2019 Jul;8(13):e1900136. doi: 10.1002/adhm.201900136. Epub 2019 Apr 15.

Abstract

Multidrug resistance is one of the leading causes of chemotherapy failure in cancer patients. Early detection and capture of drug-resistant tumor cells can facilitate the monitoring of the therapy process and improve the prognosis of patients. In this study, novel P-glycoprotein (P-gp) antibody modified porous hydrogel particles are proposed for drug-resistant tumor cells capture. The hydrogel particles employ a highly biocompatible hydrogel, methacrylate gelatin (GelMA), as the carrier and replicate from the silica colloidal crystal beads. By the modification of P-gp antibody probes on their surfaces, the hydrogel particles are endowed with the ability to capture drug-resistant tumor cells, which overexpress specific components of P-gp on their membranes. Additionally, the acquired ordered porous nanostructure of the particles can provide not only more surface area for antibody immobilization but also a nanopatterned platform for highly efficient target cell capture. The above advantages make the porous hydrogel particles ideal for efficient capture and detection of the drug-resistant tumor cells, which can be expected to facilitate the point-of-care pharmacotherapy and promisingly improve the patient outcomes.

Keywords: P-glycoprotein (P-gp); colloidal crystals; leukemia; multidrug resistance (MDR); porous particles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B / immunology*
  • Antibodies, Neoplasm / chemistry*
  • Antibodies, Neoplasm / immunology
  • Antibodies, Neoplasm / metabolism
  • Antibodies, Neoplasm / pharmacology
  • Cell Survival / drug effects
  • Drug Resistance, Neoplasm* / drug effects
  • Gelatin / chemistry
  • Humans
  • Hydrogels / chemistry*
  • K562 Cells
  • Methacrylates / chemistry
  • Nanostructures / chemistry
  • Neoplasms / metabolism
  • Neoplasms / pathology*
  • Porosity
  • Silicon Dioxide / chemistry

Substances

  • ATP Binding Cassette Transporter, Subfamily B
  • Antibodies, Neoplasm
  • Hydrogels
  • Methacrylates
  • Silicon Dioxide
  • Gelatin