Biomaterials based on the modulation of macrophages have gained increased attention recently. Macrophages are generally divided into the pro-inflammatory M1 and pro-regenerative M2 phenotypes. Macrophages play a pivotal role in bone regeneration by regulating osteoblast differentiation and secreting pro-regenerative factors. In the present study, epigallocatechin-3-gallate (EGCG)-modified collagen membranes downregulated the expression of inflammatory factors and promoted the recruitment of M2 macrophages, as evidenced by the expression of M2 macrophage markers (CD163 and CD206). It is further demonstrated that the recruitment of M2 macrophages may be involved with CC chemokine receptor type 2 (CCR2) signaling, with a significant downregulation of CD206 following CCR2 knockout. These results suggested that EGCG-modified collagen membranes may modulate the recruitment of macrophages and can be applied to guided bone regeneration and guided tissue regeneration.
Keywords: Epigallocatechin-3-gallate; Extracellular matrix; Macrophage; Polarization; Pro-regenerative immune system.
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