Abstract
The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six intermediates in late nuclear and cytoplasmic maturation of the large subunit that reveal a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10. We also determine the structure of the biogenesis factor Yvh1 and show how it promotes assembly of the P stalk, a critical element for recruitment of GTPases that drive translation. Together, our structures provide a blueprint for final assembly of a functional ribosome.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Cryoelectron Microscopy
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Dual-Specificity Phosphatases / chemistry
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Dual-Specificity Phosphatases / metabolism
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Models, Molecular
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Nucleic Acid Conformation
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Peptidyl Transferases / chemistry
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Peptidyl Transferases / metabolism
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Protein Conformation
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RNA, Fungal / chemistry
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RNA, Fungal / metabolism
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RNA-Binding Proteins / chemistry
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RNA-Binding Proteins / metabolism
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Ribosomal Proteins / chemistry*
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Ribosomal Proteins / metabolism*
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Ribosome Subunits, Large, Eukaryotic / chemistry
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Ribosome Subunits, Large, Eukaryotic / metabolism
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Ribosome Subunits, Large, Eukaryotic / ultrastructure
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Ribosomes / chemistry*
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Ribosomes / metabolism*
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Ribosomes / ultrastructure
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae / ultrastructure
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Saccharomyces cerevisiae Proteins / chemistry*
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Saccharomyces cerevisiae Proteins / metabolism*
Substances
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MRT4 protein, S cerevisiae
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NMD3 protein, S cerevisiae
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RNA, Fungal
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RNA-Binding Proteins
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RPL10 protein, S cerevisiae
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Ribosomal Proteins
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Saccharomyces cerevisiae Proteins
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Peptidyl Transferases
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Dual-Specificity Phosphatases
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YVH1 protein, S cerevisiae