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Gene. 1988 Dec 25;74(1):25-32.

Cloning type-II restriction and modification genes.

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  • 1New England Biolabs, Inc., Beverly, MA 01915.

Abstract

We have cloned into Escherichia coli the genes for 38 type-II bacterial modification methyltransferases. The clones were isolated by selecting in vitro for protectively modified recombinants. Most of the clones modify their DNA fully but a substantial number modify only partially. In approximately one-half of the clones, the genes for the corresponding endonucleases are also present. Some of these clones restrict infecting phages and others do not. Clones carrying endonuclease genes but lacking methyltransferase genes have been found, in several instances, to be viable.

PMID:
3074013
[PubMed - indexed for MEDLINE]
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