Highly selective dual sensing of ATP and ADP using fluorescent ribonucleopeptide sensors

Shun Nakano; Musashi Shimizu; Huyen Dinh; Takashi Morii

doi:10.1039/c8cc09934k

Highly selective dual sensing of ATP and ADP using fluorescent ribonucleopeptide sensors

Chem Commun (Camb). 2019 Jan 31;55(11):1611-1614. doi: 10.1039/c8cc09934k.

Authors

Shun Nakano¹, Musashi Shimizu, Huyen Dinh, Takashi Morii

Affiliation

¹ Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. t-morii@iae.kyoto-u.ac.jp.

PMID: 30657140
DOI: 10.1039/c8cc09934k

Abstract

Highly selective fluorescent sensors for ATP and ADP were constructed from RNA aptamers by applying a modular design of a ribonucleopeptide scaffold. These sensors allow facile and quantitative detection of ATP and ADP simultaneously in a solution and enable monitoring of the time-course changes of ATP and ADP concentrations in an enzymatic reaction.

MeSH terms

Adenosine Diphosphate / analysis*
Adenosine Diphosphate / chemistry
Adenosine Triphosphate / analysis*
Adenosine Triphosphate / chemistry
Aptamers, Nucleotide / chemistry*
Creatine Kinase / metabolism
Fluorescent Dyes / chemistry*
Spectrometry, Fluorescence*

Substances

Aptamers, Nucleotide
Fluorescent Dyes
Adenosine Diphosphate
Adenosine Triphosphate
Creatine Kinase