Objective: To analyze the hints role of surveillance cultures of Carbapenem-resistant Enterobacteriaceae (CRE) by perianal swabs in patients with hematological diseases, and seek risk factors of CRE bloodstream infection. Methods: The resistance of CRE from 2 914 patients with hematological diseases who cultured perianal swabs, CRE bloodstream infection and risk factors were analyzed during January 2016 to December 2017. Results: In this study, perianal swabs from 2 914 patients with hematological diseases were cultured, 74 patients were CRE positive, and bloodstream infection with CRE was found in 13 of these patients. A total of 87 CRE strains were isolated (The same patient only keep the first one for the same location), including 31 Klebsiella pheuminiae, 43 Escherichia coli, 8 Enterobacter cloacae and 6 other Enterobacteriaceae. The resistance rates to piperacillin / tazobactam, imipenem, meropenam, amikacin, levofloxacin, tigecycline were 91.9%, 74.4%, 98.8%, 17.6%, 74.4% and 8.0%, respectively. Resistance to carbapenem, aminoglycoside, quinolones and tegacycline were highly consistent between two sites from 13 patients, whose both perianal swabs and blood were positive in CRE cultures. Febrile neutropenic time, digestive tract symptoms and perianal infection were independent risk factors for bloodstream infection in patients with perianal swabs positive results, the odds ratios (OR) were 1.10 (P=0.029), 1.13 (P=0.005) and 1.23 (P=0.016), respectively. Conclusion: Perianal swabs surveillance cultures of CRE can be hints for CRE bloodstream infection in patients with hematological diseases, and also can provide suggestions for antibiotics. Long time of febrile neutropenic, digestive tract symptoms and perianal infection can be the early warning for CRE bloodstream infections.
目的： 探讨血液病患者肛周皮肤拭子细菌培养检出耐碳青霉烯类肠杆菌（CRE）对CRE血流感染的预警价值，寻找CRE血流感染的危险因素。 方法： 对2016年1月至2017年12月进行肛周皮肤拭子细菌培养的血液病患者分离出CRE的耐药率、患者后期CRE血流感染发生情况及危险因素进行回顾性分析。 结果： 2 914例血液病患者进行了肛周皮肤拭子细菌培养，74例检出CRE，其中后期发生CRE血流感染者13例（17.6%）。共分离出CRE 87株（相同患者相同部位去重），包括肺炎克雷伯菌31株、大肠埃希菌43株、阴沟肠杆菌8株、其他肠杆菌科细菌6株。分离出CRE菌株对哌拉西林/他唑巴坦、亚胺培南、美罗培南、阿米卡星、左氧氟沙星、替加环素的耐药率分别为91.9%、74.4%、98.8%、17.6%、74.4%和8.0%。13例发生CRE血流感染患者肛周皮肤拭子与血液中分离株对碳青霉烯类、氨基糖苷类、喹诺酮类及替加环素耐药程度一致率较高。粒细胞缺乏（粒缺）、存在消化道症状、肛周感染持续时间为肛周皮肤CRE阳性患者发生CRE血流感染的独立危险因素（OR值分别为1.10、1.13和1.23，P值分别为0.029、0.005、0.016）。 结论： 对血液病患者进行肛周皮肤拭子细菌培养可为后期CRE血流感染提供预警，并可为其提供抗菌药物选择参考；粒缺、伴消化道症状以及肛周感染持续时间长可作为CRE血流感染患者早期识别因素。.
Keywords: Bloodstream infection; Carbapenem-resisitant Enterobacteriaceae; Hematologic malignancies; Risk factors.