Two cellular components, PrlA and SecB, that recognize different sequence determinants are required for efficient protein export

N J Trun; J Stader; A Lupas; C Kumamoto; T J Silhavy

doi:10.1128/jb.170.12.5928-5930.1988

Two cellular components, PrlA and SecB, that recognize different sequence determinants are required for efficient protein export

J Bacteriol. 1988 Dec;170(12):5928-30. doi: 10.1128/jb.170.12.5928-5930.1988.

Authors

N J Trun¹, J Stader, A Lupas, C Kumamoto, T J Silhavy

Affiliation

¹ Department of Biology, Princeton University, New Jersey 08544.

Abstract

We exploited the conditional-lethal phenotype of secB null mutations to demonstrate that SecB function was required for PrlA-mediated suppression of signal sequence mutations. The results of these experiments provide information about the functions performed and the sequence determinants recognized by each of these components of the protein export machinery of Escherichia coli.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Bacterial Outer Membrane Proteins
Bacterial Proteins / genetics*
Escherichia coli / genetics*
Escherichia coli / metabolism
Escherichia coli Proteins*
Genes*
Genes, Bacterial*
Mutation
Phenotype
Porins
Receptors, Virus / genetics
SEC Translocation Channels

Substances

Bacterial Outer Membrane Proteins
Bacterial Proteins
Escherichia coli Proteins
Porins
Receptors, Virus
SEC Translocation Channels
SecB protein, Bacteria
SecY protein, E coli
maltoporins