Biochem Biophys Res Commun. 1988 Oct 31;156(2):931-9.

Isolation and characterization of human platelet glycoprotein IX.

Roth GJ, Ozols J, Nugent DJ, Williams SA.

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Medical Service, Veterans Administration Medical Center, Seattle, WA 98108.

Abstract

Human platelet glycoprotein IX is a small (Mr 17,000) surface glycoprotein present in the plasma membrane as a 1:1 non-covalent complex with glycoprotein Ib (Mr 165,000). Glycoprotein IX was purified to near homogeneity by sequential wheat germ agglutinin and immuno-affinity chromatography, followed by gel filtration chromatography under denaturing conditions. Purified glycoprotein IX was characterized by determination of its amino acid composition and its NH2-terminal amino acid sequence (thr-lys-asp-xxx-pro-ser-xxx-leu-thr-(thr)2-arg-ala-(leu)-(glu)-xxx-met- gly), and monospecific anti-glycoprotein IX antibody was prepared. The study outlines a useful approach for separating and characterizing glycoprotein IX, free from other members of the glycoprotein Ib complex.

PMID:: 3056407; [PubMed - indexed for MEDLINE]

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Cited by 1 PubMed Central article

Human platelet glycoprotein IX: an adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures. [Proc Natl Acad Sci U S A. 1989]
Human platelet glycoprotein IX: an adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures.
Hickey MJ, Williams SA, Roth GJ. Proc Natl Acad Sci U S A. 1989 Sep; 86(17):6773-7.

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Isolation and characterization of human platelet glycoprotein IX.
Isolation and characterization of human platelet glycoprotein IX.
Biochem Biophys Res Commun. 1988 Oct 31 ;156(2):931-9.

PubMed
Characterization of the gene encoding human platelet glycoprotein IX.
Characterization of the gene encoding human platelet glycoprotein IX.
J Biol Chem. 1993 Feb 15 ;268(5):3438-43.

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PubMed
Human platelet glycoprotein IX: an adhesive prototype of leucine-rich glycoprote...
Human platelet glycoprotein IX: an adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures.
Proc Natl Acad Sci U S A. 1989 Sep ;86(17):6773-7.

PubMed
Novel point mutation in the leucine-rich motif of the platelet glycoprotein IX a...
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Br J Haematol. 1997 Dec ;99(4):794-800.

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Isolation and characterization of human platelet glycoprotein IX.

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