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1: Clin Chem. 1988 Sep;34(9):1834-8.Click here to read Links

Protein C in human plasma determined by homogeneous enzyme immunoassay with use of a centrifugal analyzer.

Shimamoto M, Yoshimura T, Kimura N, Kita M, Hoshino N, Motoi S, Suehisa E, Nishimura K, Hayashi C, Miyai K.

Research Division, Iatron Laboratories, Inc., Tokyo, Japan.

We describe the simple and rapid enzyme immunoassay of protein C in human plasma with use of a Cobas Fara centrifugal analyzer. The antibody, labeled with horseradish peroxidase, is reacted with antigen (protein C) for 15 min. The peroxidase activity of the resulting antigen-antibody conjugate is measured at 500 nm for 5 min in the presence of excess H2O2, phenol, and 4-aminoantipyrine, as compared with that of free conjugates. Results are calculated from a stored standard curve and expressed as a percentage of the value determined for a pooled specimen of normal adult plasma. The standard curve is linear from 0% to 200%. The CV is generally less than 4% for different concentrations of protein C. In liver cirrhosis, hepatocellular carcinoma, therapy with warfarin, thrombosis, and disseminated intravascular coagulation, protein C concentrations are about 40-70% of normal. Results obtained with the present homogeneous enzyme immunoassay correlated well with those by enzyme-labeled immunosorbent assay (r = 0.97).

PMID: 3046778 [PubMed - indexed for MEDLINE]

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