The Effect of Treadmill Treatment on Oxidative Stress Markers and Endogenous Antioxidant Status in Obesity Mice

Aim: This study aimed to determine the effect of treadmill treatment on oxidative stress markers and endogenous antioxidant status seen from MDA, GSH, MnSOD enzyme specific activity and blood catalase of obese mice.

Materials and methods: This research is experimental laboratory research using post-test control design group only. The study lasted for 28 days and was divided into 4 groups of study, i.e., group K (normal control), KP (obesity control), P1 (obesity mice with 1 x 10-minute treadmill treatment a day), and P2 (obesity mice with 2 x 10-minute treadmill treatment a day).

Results: The treadmill treatment had an effect on the improvement of the oxidative status of mice with a decrease of MDA level of obesity mice blood (p ≤ 0.05) compared to KP control. An elevated endogenous antioxidant status of obesity mice was seen from elevated GSH levels, MnSOD specific activity and blood catalase of obesity mice (p ≤ 0.05) compared with KP controls. Treatment of 1 x 10-minute treadmill per day decreased blood MDA level, increased GSH enzyme and increased specific activity of MnSOD enzyme and blood catalase of obese mice.

Conclusions: The 2 x 10-minute daily treadmill did not differ significantly in improving the oxidative status and endogenous antioxidant status compared with the treadmill 1 x 10 minutes a day (p ≥ 0.05).