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Anal Biochem. 1987 Mar;161(2):559-66.

Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions.

Abstract

Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.

PMID:
3034103
[PubMed - indexed for MEDLINE]
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