Imaging SNAP-29 in Drosophila

Methods Mol Biol. 2019:1860:391-401. doi: 10.1007/978-1-4939-8760-3_26.

Abstract

SNAP-29 is expressed throughout the life cycle of fruit fly and exhibits wide tissue distribution patterns. Unlike other SNAP-25-like proteins (i.e., SNAP-25, SNAP-23/24, and SNAP-47) which primarily support exocytosis at the plasma membrane, SNAP-29 regulates various intracellular trafficking events, by partnering with proteins active in both exocytosis and endocytosis. Here we describe the protocol to localize SNAP-29 in early embryos, imaginal discs from third instar larva, and immortalized S2 cells via immunofluorescence microscopy.

Keywords: Drosophila; SNAP-29; Ubisnap; usnp.

MeSH terms

  • Animals
  • Cell Line
  • Cell Membrane / metabolism*
  • Drosophila Proteins / chemistry
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / metabolism
  • Embryo, Nonmammalian / diagnostic imaging
  • Embryo, Nonmammalian / metabolism
  • Endocytosis
  • Exocytosis
  • Fluorescent Dyes / chemistry
  • Imaginal Discs / diagnostic imaging
  • Imaginal Discs / metabolism
  • Larva / metabolism
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods
  • Models, Animal
  • SNARE Proteins / chemistry
  • SNARE Proteins / metabolism*
  • Single Molecule Imaging / instrumentation
  • Single Molecule Imaging / methods*

Substances

  • Drosophila Proteins
  • Fluorescent Dyes
  • SNARE Proteins
  • Snap29 protein, Drosophila