Sensitivity and specificity of enzyme immunofiltration and DNA hybridization for the detection of HCMV-infected cells

J Virol Methods. 1987 Feb;15(2):109-20. doi: 10.1016/0166-0934(87)90054-1.

Abstract

The sensitivity and specificity of enzyme immunofiltration and DNA hybridization were compared in human cytomegalovirus (HCMV) (AD 169)-infected MRC-5 cells. The enzyme immunofiltration was carried out on glass fiber filters in microplates, using an HCMV (AD 169) monoclonal antibody and a peroxidase conjugate. The DNA hybridization was carried out with a microfiltration apparatus, using a 32P-labelled HCMV (AD 169) Eco R1 D fragment probe. The sensitivities of enzyme immunofiltration and DNA hybridization were 1.82 X 10(3) and 1.13 X 10(3) infected cells, respectively. Both methods were highly specific, but enzyme immunofiltration was faster and simpler.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology
  • Antigens, Viral / analysis*
  • Autoradiography
  • Cell Line
  • Cytomegalovirus / genetics
  • Cytomegalovirus / immunology
  • Cytomegalovirus / isolation & purification*
  • DNA, Viral / analysis*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Immunoenzyme Techniques
  • Immunosorbent Techniques*
  • Nucleic Acid Hybridization*
  • Predictive Value of Tests
  • Regression Analysis

Substances

  • Antibodies, Monoclonal
  • Antigens, Viral
  • DNA, Viral