MicroRNA-590-5p suppresses the proliferation and invasion of non-small cell lung cancer by regulating GAB1

Eur Rev Med Pharmacol Sci. 2018 Sep;22(18):5954-5963. doi: 10.26355/eurrev_201809_15926.

Abstract

Objective: Some specific microRNAs (miRNAs) have been identified to regulate the tumorigenesis of non-small cell lung cancer (NSCLC). MiR-590-5p was found to involve in the carcinogenesis of human cancers. This study aims at exploring the role of miR-590-5p in the pathogenesis of NSCLC.

Patients and methods: The expressions of miR-590-5p and GAB1 were measured by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blot, respectively. The biological functions of miR-590-5p and GAB1 on cell viability and invasion were investigated through MTT and transwell assays. The binding site between miR-590-5p and GAB1 was verified by dual-luciferase reporter gene assay (DLR).

Results: MiR-590-5p expression was downregulated in NSCLC. MiR-590-5p overexpression inhibited the proliferation and invasion of NSCLC cells. Furthermore, miR-590-5p was confirmed to directly target GAB1. GAB1 knockdown had the same effect as overexpression of miR-590-5p in NSCLC. Moreover, overexpression of GAB1 partially reversed the suppressive effect of miR-590-5p on NSCLC.

Conclusions: MiR-590-5p suppressed cell proliferation and invasion of NSCLC by inhibiting GAB1 expression, indicating that miR-590-5p was a suppressive miRNA in NSCLC.

MeSH terms

  • 3' Untranslated Regions
  • A549 Cells
  • Adaptor Proteins, Signal Transducing / genetics*
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Down-Regulation
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lung Neoplasms / genetics*
  • Male
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness

Substances

  • 3' Untranslated Regions
  • Adaptor Proteins, Signal Transducing
  • GAB1 protein, human
  • MIRN590 microRNA, human
  • MicroRNAs