Fabrication of an electrochemical biosensor with ZnO nanoflakes interface for methylglyoxal quantification in food samples

Amudha Jayaprakasan; Amarnath Thangavel; Lakshmishri Ramachandra Bhat; Manju Bhargavi Gumpu; Noel Nesakumar; K Jayanth Babu; Srinivasan Vedantham; John Bosco Balaguru Rayappan

doi:10.1007/s10068-017-0193-0

Fabrication of an electrochemical biosensor with ZnO nanoflakes interface for methylglyoxal quantification in food samples

Food Sci Biotechnol. 2017 Dec 2;27(1):9-17. doi: 10.1007/s10068-017-0193-0. eCollection 2018 Feb.

Authors

Amudha Jayaprakasan^#^{1

2}, Amarnath Thangavel^#^{1

2}, Lakshmishri Ramachandra Bhat^{1

3}, Manju Bhargavi Gumpu^{1

3}, Noel Nesakumar^{1

3}, K Jayanth Babu^{1

2}, Srinivasan Vedantham^{1

2}, John Bosco Balaguru Rayappan^{1

3}

Affiliations

¹ 1Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA University, Thanjavur, Tamil Nadu 613 401 India.
² 2School of Chemical & Biotechnology, SASTRA University, Thanjavur, Tamil Nadu 613 401 India.
³ 3School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu 613 401 India.

^# Contributed equally.

Abstract

Increased consumption of fried foods such as grilled chicken contains elevated levels of methylglyoxal (MG), which is associated with diabetes mellitus. Hence, in this work, glyoxalase 1(GLO 1) based, zinc oxide (ZnO) flakes interfaced mediator free electrochemical biosensor was developed to detect MG in grilled chicken. ZnO flakes were synthesized by direct precipitation method. X-ray diffractometer and field emission scanning electron microscope were used to study the structural and morphological characteristics of ZnO flakes. The immobilization of GLO 1 on Pt/ZnO flakes modified electrode was confirmed by Fourier transform infrared spectroscopy. Cyclic voltammetric and amperometric studies were carried out using Pt/ZnO flakes/GLO 1 working electrode. The developed biosensor exhibited linear range of 0.6-2.0 µM, sensitivity of 0.281 µA µM^-1, LOD of 9 nM with a response time of <4 s and shelf life of 18 days (89%).

Keywords: Amperometry; Biosensor; Cyclic voltammetry; Human glyoxalase 1; Methylglyoxal; ZnO flakes.