To investigate if oxymatrine could ameliorate hippocampus ischemia/reperfusion (I/R) injury induced in rats and explore the possible mechanism. Rats were randomly divided into four groups: sham group, I/R group, I/R + OMT-treated group, I/R + Vehicle-treated group. Oxymatrine or vehicle solution was intraperitoneally injected OMT (150 mg/kg) 60 min before renal ischemia respectively. Water maze was measured; cell apoptosis was assessed by doing TUNEL assay and detecting the expression of P53, Bax, and Cleaved-Caspase-3; autophagy were assessed by measuring the expression of LC3 and P62. The expression of SIRT1 was also detected. Oxymatrine treatment alleviated histological injury in I/R rats, inhibiting apoptosis, promoting autophagy and accompanied by upregulated expression of SIRT1 proteins. Oxymatrine may attenuate hippocampus ischemia/reperfusion injury through upregulation SIRT1, further influencing the processes of apoptosis and autophagy.
Keywords: Bax; Hippocampus; Ischemia/reperfusion; LC3; Oxymatrine; SIRT1; p53.
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