The abundance and diversity of ammonia-oxidizing microorganisms in sediments of an aquacultural area of Eastern Lake Taihu were investigated. Real-time quantitative PCR was used to analyze the abundance of archaeal and bacterial amoA genes. Cloned libraries were constructed to investigate the structure and diversity of the microbial community. By comparing community characteristics of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in different zones, we found that:①Copy numbers of the bacterial amoA gene outnumbered those of archaeal amoA genes in the aquacultural zone; ②Diversity of AOA and AOB was higher in the aquaculture zone and control zone, respectively; ③ The dominant cluster of AOA and AOB in both sediments of aquiculture zone and control zone was Nitrosopumilus and Nitrosospira respectively. It was therefore the community structure of AOA (rather than AOB) in lake sediments that was affected by aquacultural activity.
Keywords: Eastern Lake Taihu; ammonia-oxidizing archaea; ammonia-oxidizing bacteria; aquaculture; sediment.